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2.5. Immunofluorescence Staining of Colon

BY Bo Yang
ML Mingjie Li
SW Shuo Wang
RR R. Paul Ross
CS Catherine Stanton
JZ Jianxin Zhao
HZ Hao Zhang
WC Wei Chen
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For immunofluorescence analysis, the cut sections were stained with 200 times dilution of ZO-1 (AB96587; Abcam, Cambs, UK), 100 times dilution of Claudin3 (AB15102; Abcam, Cambs, UK), and 100 times dilution of Occludin (AB216327; Abcam, Cambs, UK). All the antibodies were anti-rabbit. Slides were examined and analyzed using an epifluorescence microscope.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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