Lentivirus LV-EF1α-hTau-GFP (3.50E+8 vg/mL) was packaged by OBiO (Shanghai, China) and used for hTau-overexpression (fused with GFP) in cultured primary rat neurons. AAV-CaMKIIα-Cre (diluted to 5.21E + 07 vg/mL) and AAV-EF1α-DIO-mCherry (3.24E + 12 vg/mL) were obtained from BrainVTA (Wuhan, China) and co-injected (1:1 vol/vol) for sparse labeling of dorsal DG neurons. Doxycycline was purchased from Energy Chemical (Shanghai, China). Rabbit polyclonal DEPTAC antibody was prepared by Daian Biotechnology (Wuhan, China). All other reagents including MG132 were purchased from Sigma-Aldrich (USA), unless otherwise specified.
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