2.5. Synthesis of Anti-FB1 Monoclonal Antibodies Labeled GNB129 (GNB129–mAbs)

GNB₁₂₉–mAbs were prepared through the formation of a peptide bond between the carboxyl group of GNB₁₂₉ and the amino group of antibodies by using the active ester method. Approximately 1 µL anti-FB₁ mAbs (6.4 mg/mL) was added into 400 µL 0.01 M pH 6.5 phosphate buffer (PB) solution containing GNB₁₂₉ (0.14 pM) and 1-ethyl-3-(3-dimethylamino) propyl carbodiimide (EDC) (1 µg). The mixed solution was incubated for 90 min at room temperature. Subsequently, 10 mg BSA was added into the mixture solution for another 1 h of reaction. The synthesized GNB₁₂₉–mAbs were then centrifuged and resuspended in 200 µL 0.01 M PB (pH 7.4) containing 25% sucrose, 1% bovine serum albumin (BSA), and 0.1% sodium azide.