2.6.1. Degreasing treatment

Peanut kernels contain as much as 43%–52% fat. To reduce interference in subsequent experiments, the freeze-dried, powdered peanuts/peanut sprouts were defatted, and the protein was extracted. One gram of powder from each treatment was mixed with 10 ml acetone and stirred at 4 °C for 2 h. Mixtures were then centrifuged at 3000 g (TGL-10G, Shanghai Anting Scientific Instrument Factory Co., Ltd., China) for 10 min, and the precipitate was obtained. The precipitate typically required three to four rounds of degreasing until the supernatant was transparent. The precipitate was then naturally air dried in the fume hood.