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Isolation of IgG from human plasma
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IgG was isolated from plasma samples by affinity chromatography as described previously [30]. In brief, IgG was isolated in a high-throughput manner, using 96-well protein G monolithic plates (BIA Separations, Slovenia), starting from 100 μl of plasma. Plasma was diluted 7× with phosphate buffered saline (PBS; Merck, Germany) and applied to the protein G plate. IgG was eluted with 1 ml of 0.1 M formic acid (Merck, Germany) and immediately neutralised with 1 M ammonium bicarbonate (Acros Organics, USA).
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