Macro and Micromorphological Features of the A. niger Environmental Isolates

The macromorphological features, i.e., the diameter of colonies and degree of sporulation, were observed visually on the fifth day of cultivation on SDA in Petri dishes and had a diameter of 6 cm in three repetitions. The micromorphology figures were made with a Canon IXUS 16.1 megapixels camera (Japan). Also, micromorphological features (Figure 2) were observed under an Axio Scope A1 Carl Zeiss Jena light microscope on the fifth day of cultivation in a drop of lactic acid enriched with a cotton blue stain (0.01%).

Macromorphological features of Aspergillus niger environmental isolates on the fifth day of cultivation on SDA agar in Petri dishes with a diameter of 6 cm. Colonies form different diameters and also sporulation depending on environmental contamination (left side). Micromorphological features of Aspergillus niger environmental isolates on the fifth day in a drop of lactic acid enriched with cotton blue stain (0.01%). The figures show deformations of conidiophores, such as swelling, narrowing or deformation of the conidial head (vesiculum). We assumed that these deformations can be caused by long term contaminated environment (right side).