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miRNA Target Prediction and Target Function Analysis

SQ Shijie Qin
JX Jieyun Xu
YY Yunmeng Yi
SJ Sizhu Jiang
PJ Ping Jin
XX Xinyi Xia
FM Fei Ma
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These target prediction results of miRNAs were derived from miRWalk3.0 (http://mirwalk.umm.uni-heidelberg.de/), which comprehensively contain the miRNA target genes of humans, mice and other species through integrating the TarPmiR algorithm and the information from miRDB, TargetScan, miRTarBase and other databases (33, 34). Since miRNAs negatively regulate their target genes, the spearman correlation coefficient and significance between miRNA and their target genes were calculated. Only those targets with a significant negative correlation with miRNAs (r <−0.2, p <0.05) were further considered as true targets of miRNAs. Kyoto encyclopedia of Genes and Genomes (KEGG) enrichment analysis of targets was performed using the clusterProfiler package (35).

Copyright and License information:  ©2021 Qin, Xu, Yi, Jiang, Jin, Xia and Ma
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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