Growth Curve

To study the growth curve, a 96-well microtiter plate was used. Bacterial cultures grown for 16 h, 28°C were diluted to a final concentration of 1 × 106 CFU in 200 μL of fresh LB containing non-lethal concentration of the compounds (or no compound, for the control) in a Tecan Spark^® multimode microplate reader (Tecan Trading AG, Switzerland) for 24 h. The plates were incubated at 28°C and cell density was measured at 600 nm every hour for 24 h, using a Tecan Spark^® multimode microplate reader (Tecan Trading AG, Switzerland). Each experiment was repeated at least twice with four replicates for each compound. Serial dilutions were made and plated onto LB agar plates for bacterial cell counting.