Cell proliferation assays

Cell proliferation assays were performed 24 h after transfection. For CCK8 viability assay, cells were seeded at a density of 2,000 cells/well in a 96-well plate. 10% CCK8 (Beyotime, Suzhou, China) was added to the well, and the absorbance at 450 nm was measured every 24 h. For EdU assay, 8,000 cells/100 μL were plated in 96-well plates. One hundred microliters of 50 μM EdU solution (EdU Apollo488 In Vitro Imaging Kit, RiboBio, Guangzhou, China) was incubated with cells. EdU was stained with red fluorescence, and images were photographed by fluorescence microscope to count the proportion of proliferating cells.