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Male C57BL/6J mice were administered dexamethasone (Dex, Sigma, St Louis, MO, USA, catalog no. D1159) intraperitoneally (i.p.) at a dose of 10 mg/kg dissolved in saline. The injection volume was 10 μl/g body weight. Vehicle treated mice were injected with the same amount of saline. Injections were performed between 08:00 am and 09:00 am. 4 h after the injection, all mice were sacrificed by decapitation following quick anesthesia by isoflurane. Brains were removed, snap-frozen in isopentane at −40°C, and stored at −80°C until further processing.
Copyright and License information: ©2021
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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