4.5. Ion Content Measurements

Concentrations of sodium (Na⁺), potassium (K⁺), and chloride (Cl⁻) were measured in the roots and leaves of plants sampled after the salt treatments, and in the corresponding non-stressed controls, according to Weimberg [85]. Dried material (ca. 0.1 g) was ground to a fine powder and extracted in 15 mL of MilliQ water, incubating the samples for one hour in a water bath, at 95 °C, followed by cooling to room temperature and filtration through a 0.45 μm Gelman nylon filter (Pall Corporation, Port Washington, NY, USA). The cations Na⁺ and K⁺ were quantified with a PFP7 flame photometer (Jenway Inc., Burlington, VT, USA) and the anion using a chlorimeter (Sherwood, model 926, Cambridge, UK).