GO and KEGG Pathway Analysis

The DAVID database (version 6.8),³ as a bioinformatic database that integrates biological data and analysis tools to provide systematic and comprehensive biological annotation information for large-scale gene or protein lists, was used to perform gene enrichment analysis of the collection of the potential target genes of the DE-miRNAs (Dennis Sherman et al., 2003). We have taken a collection of the predicted target genes of the three target miRNAs respectively to obtain the gene list. On the background of the human genome-wide annotation data (org.Hs.eg.db), we utilized the cluster profiler package of the R language (version: 3.6.5) to perform the enrichment analysis of GO and KEGG pathways. The p-value significance threshold is set to 0.5, and the q-value significance threshold is set to 0.2. GO functional annotation analysis and KEGG pathway analysis based on the DAVID database were conducted on the candidate target genes of the DE-miRNAs. P-value < 0.05 was regarded as statistically significant.