Prediction of Target Genes of the DE-miRNAs

XD Xiaotong Duan
SQ Simiao Qiao
DL Dianhe Li
SL Shangbiao Li
ZZ Zhihao Zheng
QW Qin Wang
XZ Xiaoxia Zhu
request Request a Protocol
ask Ask a question
Favorite

The V7.0 miRTarBase,2 which is an online database containing plenty of experimentally validated microRNA–target interactions (Hsu et al., 2011), was applied to identify the potential target genes of the DE-miRNA. Species selects: Homo sapiens. miRNA ID was entered as “hsa-miR-590-3p/hsa-miR-492/hsa-miR-631” to obtain the gene list of predicted target genes. Since the list contains a small number of strong evidence genes, we selected all the genes in the gene list (including strong evidence and less strong evidence) for relevant pathway enrichment analysis.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A