The V7.0 miRTarBase,2 which is an online database containing plenty of experimentally validated microRNA–target interactions (Hsu et al., 2011), was applied to identify the potential target genes of the DE-miRNA. Species selects: Homo sapiens. miRNA ID was entered as “hsa-miR-590-3p/hsa-miR-492/hsa-miR-631” to obtain the gene list of predicted target genes. Since the list contains a small number of strong evidence genes, we selected all the genes in the gene list (including strong evidence and less strong evidence) for relevant pathway enrichment analysis.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.