2.3. Experimental Schedule

KN Kaho Nakamura
ET Eri Tajiri
YH Yoichi Hatamoto
TA Takafumi Ando
SS Seiya Shimoda
EY Eiichi Yoshimura
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Each participant wore a continuous blood glucose measuring device and a tri-axis accelerometer from the day before each 3-day experimental period began and throughout the 3-day period. The meals for day 1 of the experiment were provided the day before the experimental period began, and were consumed the following day at 8:00, 12:00, and 18:00 or 21:00. Participants recorded meal intake using a paper diary. On day 2, the participants visited the laboratory before breaking their fast to have their height and weight measured and collect their meals for that day. In addition, the participants’ subjective psychological status was evaluated by questionnaire (self-reported visual analog scores for various psychological factors) before breakfast, 30 min after breakfast, before lunch, 30 min after lunch, before dinner, 30 min after dinner, and at 23:00. On the third day of the experiment, the participants similarly visited the laboratory under fasting conditions, had their height and weight measured, and collected their meals for that day. In addition, the psychological questionnaire was filled out before breakfast and 30 min after breakfast. Before breakfast, after resting for 30 min the resting metabolic rate was measured for 10 min in the seated position. Participants were instructed to start eating breakfast at 8:00 and finish eating by 8:15. Then, the participants’ resting metabolism was evaluated by exhaled gas analysis (15 min, 30 min, 1 h, 2 h, 3 h, and 4 h after finishing breakfast). The participants remained at the laboratory control until all metabolic measurements were completed (until 12:30). The continuous blood glucose monitor and accelerometer were removed at the end of the 3-day experimental period. Day 1 was set as an intervention adjustment period to decrease any acute effects. Data from Day 2 and Day 3 of intervention were used in the analysis.

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