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Quantitative reverse transcription polymerase chain reaction (qPCR)
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Total RNA was extracted from IECs, SCs, and BM-MSCs by using an RNeasy Mini Kit (Qiagen, Valencia, CA) with on-column DNA digestion to eliminate genomic contamination. cDNA was synthesized by using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). Real-time PCR was conducted by using SYBR Green PCR Master Mix (Applied Biosystems, Thermo Fisher Scientific). The primer sequences used are shown in Table 2. The fold changes in expression were calculated using the 2−ΔΔCt method [28].
Primer sequences used for qPCR
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