C. elegans strains and maintenance

Nematode strains were maintained according to standard methods [77,78]. Strains were maintained on NGM plates with 150 μl OP50 bacteria at 20°C. N2 (wild-type) and git-1 animals were transferred into separate plates at the late L4 stage using a dissecting scope (‘Christmas tree’ stage vulva, appearing at late L4-very early young adult, as confirmed by Nomarski optics), and imaged 24 hours later, being one-day adults. For S7 Fig, BP2117 animals were either imaged at late L4 stage (defined as described above) or as young adults, defined by presence of first 1–10 eggs ordered in a single row, as confirmed by Nomarski optics. In addition to C. elegans strain N2 the following strains were used: BP709 [hmnIs133 (ser-2prom3::kaede)] [22], RB1540 git-1(ok1848) X, BP1054 git-1(ok1848) X; hmnIs133 (ser-2prom3::kaede). BP1077 git-1(tm1962) X; hmnEx133(ser-2prom3::kaede), (a non-mutant hmnIs133 (ser-2prom3::kaede) sibling of the cross which derived BP1077 was utilized as WT), BP2117 dzIs53[pF49H12.4::mCherry]; hyEx372[pmyo-2::GFP, pdes-2::nhr-25,bluescript]. git-1(ok1848) was generated by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation which is part of the International C. elegans Gene Knockout Consortium. git-1(tm1962) was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium. BP1077 and BP1054 were generated by crossing BP709 with the git-1 alleles and the genotypes were confirmed by PCR.