An FKBP-ABL retroviral vector was constructed as described previously (25). HEK293T cells were co-transfected with an empty vector control (Mock) or pMx-FKBP-ABL with pSV and pVSVG using the CalPhos Mammalian Transfection Kit (Clontech). Saos-2 cells were infected as described previously (25).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.