Establishment of Glioma Stable Cell Lines

For real time monitoring of cell viability, D54 and U87MG were transduced with retrovirus expressing effluc (Enhanced firefly luciferase)³¹ and sorted using a flow cytometer. The stable glioma cells expressing effluc were referred to as D54/effluc or U87MG/effluc cells. To monitor apoptosis in real-time, a D54 expressing caspase-3 sensor stable cells line was used that had been established in our previous studies.^32,33 Briefly, D54 cells were transfected with the caspase-3 sensor construct using Fugene 6 (Roche), and subjected to known concentration of G418 for stable clone selection. The stable D54 cells expressing the caspase-3 sensor were referred to as D54/C cells.