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For determination of H2O2, 0.15 g fresh leaves were homogenized in 0.1% TCA and then centrifuged for 15 min at 12000 × g, the supernatant mixed with 10 mM potassium phosphate buffer (pH 7.0) and 1 mL potassium iodide (1 M). H2O2 utilized to establish a standard curve. Absorbance at 390 nm were used to estimate the H2O2 55 by using the UV-VIS spectrophotometer (Hitachi U–2910).
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