Huh-7 cells at logarithmic growth stage were inoculated into a 96-well plate with density of 1 × 104 cells/well. After indicated transfection for 24 h, the supernatant of the cells in each group was taken, and the levels of glucose and lactate in the culture supernatant was, respectively, detected with the glucose assay kit (Elabscience) and lactate assay kit (Elabscience).
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