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Micro-computed tomography

YL Yifei Liu
XF Xinzeng Feng
HL Hao Liu
DM David W. McComb
CB Christopher K. Breuer
MS Michael S. Sacks
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μCT imaging was done at the Center for Electron Microscopy and Analysis (Columbus, OH) using a Heliscan μCT (Thermo Fisher Scientific). Samples were scanned with a voxel size of 2.8 μm. Reconstruction was done in sequence with a filtered back projection algorithm. Volume rendering and image visualization was done in Avizo (Version 2020.2; Thermo Fisher Scientific) to determine locations of interest. Once a region of interest was located, the sample block was trimmed using ultramicrotomy. Using anatomical features as markers, we manually registered the virtual cross-section provided by the μCT volume rendering with the progressive cross sections of the sample via optical microscope. By comparing features on the specimen surface to the μCT cross-section, we ensure that we are along the desired slicing direction.

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