2.8. Controls

An inert monoclonal control [Bioscot‐ Millipore] was the negative antisera control used with each set of antisera prepared.

Each profile was controlled with the relevant controls placed throughout each run. ²¹

ABOa RBC controls were: A₂B, A₁, B, weak RhD+, and DVI+ which were prepared from RBC packs daily, washed and resuspended in saline. An R1r K+ RBC (a previous donation with historical phenotype) was also necessary.

Anti D [Quotient‐Albacheck; 0.3 IU/mL] was the sensitivity control for antibody screening. An anti‐ A,B [Bioscot‐millipore; ES‐15/ES‐4] prepared at 1:16 dilution was used as a control for donor anti‐ A,B high titer detection.

ABOb used the same controls as ABOa except no requirement for a K+ cell; ABOb profile also had a group A, B, and O (RBC controls selected from a previous ABOb run where strong reverse group reactions were observed) to control the reverse ABO.

The Rh phenotype profile had the following controls: R₁R₁, R₂R₂, R₁R₂, R₁r, R₂r, R_or, r′r, r″r and rr, where using Fisher‐Race terminology; R₁ = DCe, R₂ = DcE, R_o = Dce, R_z = DCE, r′ = dCe, r″ = dcE, and r = dce. ¹³

The JK profile was controlled with: two Jk(a−b+), two Jk(a+b−), and two Jk(a+b+) controls. RBC controls for the Rh phenotype and Kidd profiles were selected from previous testing / historical donor phenotypes.