Fing Mapping of Gene RsPP

To validate the BSA-seq results and further narrow down the region containing the target gene, 50 pairs of Indel primers were developed according to the comparative genomic information of the P-pool and W-pool (Supplementary Table 1). The primers showing sufficient polymorphism were further used to genotype the F₂ population plants. PCR amplification of molecular markers and gel electrophoresis were conducted as described previously (Liu et al., 2017). Sequences of primers used for mapping are listed in Supplementary Table 1.