According to the median value of the risk score in the training set as the risk cutoff value, patients with a high risk score were divided into a high-risk group and those with a low risk score were divided into a low-risk group. Next, based on RNA profiles, “CIBERSORT” package was used to quantify 22 immune cells infiltration levels in colon cancer samples with cutoff P value < 0.05. Further analyses were performed to explore the relationships between prognostic risk, somatic mutation, MSI, single gene mutations and immune cell infiltration level.
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