4.4. Antibody-Dependent Cellular Cytotoxicity (ADCC) in Cell Lines

THP-1, MV4-11, HL-60, CHO-FRβ, and CHO-K1 cells transduced with GFP-2A-fLUC lentiviral vector were used in ADCC assays. 1 × 10⁴ cells were plated in triplicate in a 96 well plate in 100 μL phenol-free complete media. Adherent cell lines were plated the day prior to the experiment to allow for adherence and fresh media exchanged on the day of the experiment. m909 was added at concentrations indicated in each study and incubated with cells for 20 min. NK cells were then added at an E:T ratio of 10:1 and co-cultured overnight. Residual luciferase activity was then measured using the Extended-Glow Luciferase Reporter Assay System (Life Technologies, Carlsbad, CA, USA). Percent of lysis was calculated as follows: (100 − ((average signal antibody-treated wells)/(average signal cells alone) × 100)).