All the experiments were performed following directive 2010/63/UE on the protection of animals used for scientific purposes and in line with Italian legislation and approved by the Ethics Committee of the Edmund Mach Foundation (n. 99F6E.0). The study protocol was authorized by the Italian Ministry of Health (530/2018-PR). In order to avoid animal suffering, fish were euthanized by immersion in a lethal dose solution of tricaine methanesulfonate (MS-222, 1 g L−1) before tissue sampling.
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