Analysis of cell viability through MTT assay

The influence of AS on the viability of human colon cancer (SW620, HCT116, and HT-29) cells was examined via the MTT method [74]. Briefly, 3 × 10⁴ cells per well were grown in 24-well plates and treated with different amounts of AS (0–300 μg/mL) for 24 h. Earlier to AS treatment, 400 μL of 0.5 mg/mL MTT in PBS was incubated in each well. After culturing at 37°C for 2 h, 400 μL of DMSO were used to dissolve up the MTT formazan crystals, and the absorbance was monitored at 570 nm (A570) by utilizing ELISA microplate reader (μ-Quant, USA). Cell viability rate (%) was determined as (A570 of treated cells/A570 of untreated cells) × 100. The experiments were carried out in triplicates.