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This test followed a standard protocol.30,31 Three hours after P. berghei infection, treatment commenced on the first day and continued for the next 3 days once daily (D0–D3). Parasitemia level and percentage parasitemia suppression were determined by taking blood from the tail of each mouse on the fifth day (D4).27 Each mouse was also observed for 28 days after treatment to determine mean survival time. Weight, temperature, and packed-cell volume (PCV) were determined just before infection and on the fifth day (D4).
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