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Leghemoglobin in the soybean nodules was determined by using the modified method of Wilson and Reisenauer (1963). The leghemoglobin was extracted with Drabkin’s solution and measured colorimetrically as the CMLHb complex after centrifuging. The leghemoglobin content (mg g−1) of each sample was determined directly from the calibration curve.
Copyright and License information: ©2021 Halwani, Reckling, Egamberdieva, Omari, Bellingrath-Kimura, Bachinger and Bloch.
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