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TFK-1 and HCCC9810 cells were plated on 96-well plates at a density of 50, 000 cells/well and treated with different doses of galangin (12.5, 25, 50, 75, 100, 150, 200, 300, 400 μM) for 24 hours. After treatment, 10 μl CCK-8 (CK04, DOJIDO) was added into each well and incubated for 2 hours. The absorbance was measured at 450 nm using an ELISA plate reader (DTX880, Beckman).
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