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2.2. Surface plasmon resonance (SPR) experiment

YL Yingyi Long
SS Shuyi Song
FL Feiyang Luo
XH Xiaojian Han
CH Chao Hu
YW Yingming Wang
SL Shenglong Li
WW Wang Wang
HZ Huajun Zhang
BZ Bo Zhang
TL Tingting Li
AJ Aishun Jin
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The affinity between the mAb and SARS-CoV-2 RBD was measured by SPR. The anti-human Fc antibody was immobilized on the CM5 chip (GE Healthcare, USA) to capture antibodies. After the binding of detected mAb by the chip, the gradient concentrations of SARS-CoV-2 RBD were flowed through the sensor chip system. The affinity of mAb was calculated by 1:1 binding model with Biacore X100 evaluation software (version 2.0.2).

In order to determine the inhibition rate of mAbs on the ACE-RBD interaction, SARS-CoV-2 RBD was loaded on CM5 sensor chip about to 250 RUs. Then ACE2 (20 μg/mL) was injected for 60 s in the present or absence of the mAbs. The blocking efficacy was determined by comparing the response units with and without prior antibody incubation.

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