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Emulsification Index (%E24)
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The emulsification index (%E24) was analyzed, as reported earlier (Elshafie et al., 2015). Equal concentration of biosurfactant (2 ml) was added with different hydrocarbons (olive oil, crude oil and mineral oil) and vortexed at maximum speed for 3 min and kept at room temperature for 192 h. The emulsions formed were differentiated with negative control, i.e., YM broth, and positive control, i.e., Tween-20.
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