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Raw 2 × 300 MiSeq reads from the paired VH:VL library were trimmed based on sequence quality29 and submitted to MiXCR for gene annotation.30 Productive VH and VL reads were split by isotype and paired using a custom Python script. Sequences with ≥2 reads were grouped into lineages by clustering the CDR-H3 region on 90% nucleotide identity.
Copyright and License information: ©2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.
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