Viscometric determinations of the average degree of polymerization (average DPv) were performed according to the standard viscometric methods [17,30], using a glass capillary viscosimeter (Schott and Gen, Mainz, Germany) and fresh CED solvent (Carlo Erba Reagents S.r.l., Milan, Italy). Twenty milligrams of paper was dissolved in 20 mL of solution, consisting of 10 mL cupriethylenediamine (CED) and 10 mL deionized water. The efflux time of a solution was measured in duplicates. The Mark–Houwink–Sakurada equation was used to calculate average DPv [31].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.