Viral transductions (spinfection)

All virus transductions were performed using established spinfection protocols. Briefly, cells were seeded in 6 well/24 well plates. Twenty-four hours postseeding, wells were washed and fresh media was added (1.5 mL/500 µL respectively). The required virus was diluted into fresh media (500 µL/250 µL respectively) and polybrene was added to a final concentration of 4 µg/mL. Virus/polybrene mixture was gently mixed and added dropwise onto the cells. Plates were spinfected by centrifugation at 1200 g for 1.5 h at 30°C (Sorvall LYNX 4000, Thermo Scientific, cat#; 75006580). Plates were returned to a humidified 5% CO₂, 37°C cell culture incubator post spinfection. Cells were supplemented with 1 mL of fresh media 48 h postspinfection. Cells were split 72 h postspinfection and expanded for downstream applications (selection/FACS).