Statistical analyses were conducted using GraphPad Prism 5 software. Data was tested for the normality by the D'Agostino–Pearson omnibus normality test and similar variance by F-test. Comparison between any two groups was by two-tailed unpaired t-test for normally distributed data or non-parametric Mann–Whitney test for non-normally distributed data. Multiple group comparison was done by one-way analysis of variance for data with normal distribution. Kruskal–Wallis test was used for data with non-normal distribution. A probability value P<0.05 was considered statistically significant.
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