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Neurosphere formation assays
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Neurosphere formation assays were performed as described previously (Robledinos-Antón et al., 2017). Briefly, the neurospheres were dissociated with Accumax (Innovative Cell Technologies) into single-cell suspensions and seeded into 96-well plates at 25, 50, 75, and 100 cells/well. Cells were allowed to grow for 10–12 d, with 50 µl of fresh proliferation medium supplemented every 4 d. The number of neurospheres ≥100 µm in diameter was counted in each well, and results represent the average for six wells.
Copyright and License information: ©2021 Kulasekaran et al.
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