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2.3.3. Antimicrobial Activity by Cup Plate Agar Diffusion Method For Macro and Nanoemulsion from Anise Oil

OA Ola A. Abu Ali
ME Mehrez E. El-Naggar
MA Mohamed S. Abdel-Aziz
DS Dalia I. Saleh
MA Mohamed. A. Abu-Saied
WE Wael A. El-Sayed
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Cup plate diffusion technique was used to assess the antimicrobial actions of anise oil -based nanoemulsion by means of using the tested microbes; Gram-positive bacterium: Staphylococcus aureus (S. aureus, ATCC 6538), Gram-negative bacterium: Escherichia coli (E. coli, ATCC 25922), yeast: Candida albicans (C. albicans, ATCC 10231) and the fungus: Aspergillus niger (A. niger, NRRL A-326). Both bacteria and yeast were grown on nutrient agar medium, whereas the fungus was grown on Czapek–Dox medium [21,22]. Neomycin and cycloheximide were used as antibacterial and antifungal references, respectively.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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