Measurement Superoxide Dismutase (SOD) Activity, and Glutathione (GSH) and Malondialdehyde (MDA) Levels in Brain Tissue

ES Eunjin Sohn
YK Yu Jin Kim
JK Joo-Hwan Kim
SJ Soo-Jin Jeong
request Request a Protocol
ask Ask a question
Favorite

Intracellular SOD activity (Cat. MBS034842, MyBioSource), GSH (Cat. KA0797, Novos Biologicals, Centennial, CO, USA) level and MDA contents (Cat.10009055, Cayman, Ann Arbor, MI, USA) in brain tissues were measured according to the commercial manufacturer’s protocols. The enzyme activity and levels in supernatants separated from brain lysates were analyzed. Absorbance of reaction mixture in 96-well plate was measured using a microplate reader (Benchmark Plus, Bio-Rad).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A