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Fabrication of PLGA microparticles using microfluidic devices

CR Crystal E. Rapier
KS Kenneth J. Shea
AL Abraham P. Lee
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Microparticles are prepared from using an oil-in-water single emulsion solvent extraction process using a microfluidic droplet generator with a flow-focus design. PLGA is dissolved in non-toxic organic solvent, dimethyl carbonate (DMC). The DMC + PLGA dispersed phase solution is emulsified in a 1 wt% PVA continuous phase solution within a droplet-generating microfluidic device. The continuous and external phase channels are 50 µm in width. The flow-focusing nozzle openings for devices are 20 µm or 30 µm. A 1–10 wt% PLGA 50:50 formulation was utilized in particle formation. Microparticles are formed after the DMC solvent is extracted/diffuses from the emulsion while in aqueous solution such as PBS.

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