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ChIP-seq DNAs from the above method were diluted and subjected to qPCR interrogation. DNA was analyzed in triplicate with a 7900HT Fast Real-Time PCR System (Applied Biosystems) in a SYBR Select Master Mix (Life Technologies cat# 4472908) with the primer pairs detailed below. Cycle numbers (Ct) were determined by the default setting on Design and Analysis (Applied Biosystems) and converted to expression fold change
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