2.3.5. Swelling and Degradation Test

WL Wonseop Lim
SS Seon Young Shin
JC Jae Min Cha
HB Hojae Bae
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For swelling and degradation test, the hydrogels were prepared using the same method as described for scanning electron microscopy observation, but using the PDMS mold with different height (height of 2 mm). Bioink prepolymer solution was cross-linked under three conditions (ion, UV, and ion + UV). The prepared hydrogels were then conditioned in 1 mL DMEM (10% FBS, 1% P/S). After 24, 48, and 72 h, the liquid remaining on the hydrogel surface was removed, and the weight of the hydrogel was recorded. The swollen hydrogel was frozen at −80 °C and lyophilization was performed. The weight of the lyophilized hydrogel was recorded. The degrees of swelling and degradation were measured, respectively, by mass swelling ratio and percent mass remaining.

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