2.5.3. Hydrogen peroxide radicals scavenging assay

VN V. Uma Maheshwari Nallal
KP K. Prabha
IV I. VethaPotheher
BR Balasubramani Ravindran
AB Alaa Baazeem
SC Soon Woong Chang
GO Gloria Aderonke Otunola
MR M. Razia
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To investigate the hydrogen peroxide scavenging activity different concentrations of (2, 4, 6, 8 and 10 μg/ mL) of AB AgNPs were taken. To this 0.6 mL of 40 mM hydrogen peroxide solution was added and mixed well. After 10 min the absorbance of the reaction mixture was measured at 230 nm and Ascorbic acid was used as the standard (Ruch et al., 1989). The hydrogen peroxide radical scavenging ability of AB AgNPs was calculated as follows:

% H2O2 radicals scavenging = (Absorbance of Control–Absorbance of Sample) × 100

Absorbance of Control

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