CB1R- or CB2R-overexpressing stable cell lines

To establish stable cell lines expressing CBR, CHO-K1 cells were transfected with human CB1R (Cat. RC210397) and CB2R (Cat. SC118984) cDNA constructs (Origene, Rockville, MD, USA); the following day, media were replaced with F12 standard medium containing G418 (100 µg/ml) to select for stable clones. Two weeks later, single colonies were picked using an inverted light microscope and transferred to 24-well plates.