CellTiter-Glo cell viability assay

Cells were plated in medium (8000 cells per well, 100-μl final volume) in white, 96-well opaque plates (no. 3917, Corning). Cells were incubated for indicated intervals at 37°C in a 5% CO₂ incubator. Assay plates were removed from the incubator and equilibrated to room temperature before addition of 50 μl of CellTiter-Glo reagent (Promega), according to the manufacturer’s instructions. Plates were shaken on an orbital shaker for 2 min at 500 rpm and then incubated at room temperature on the bench top for at least 10 min. Luminescence was detected using a spectrophotometer (SpectraMax M3, Molecular Devices).