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Hematoxylin&Eosin (H&E) Staining
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The right lung tissues of the above mice were fixed with 4% paraformaldehyde at 4°C for 24 hours, dried with ethanol, and got paraffin-embedded. Next, the tissues were sliced (5 μm thick) with a slicer (Leica RM2125RT, Leica, Nussloch, Germany), and then stained with H&E using the H&E staining kit (Beyotime, Shanghai, China) according to the producer’s instructions. Sections were inspected by an Olympus BX 51 optical microscope (Olympus Corporation, Tokyo, Japan) at 200 × magnification.
Copyright and License information: ©2021 Zhang, Li, Zhao, Yang, Sang, Yan, Wang and Li
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