2.4. Total Antioxidant Activity (AOA)

The FRAP (Ferric Reducing Antioxidant Power) test system [24] was used to determine AOA. Briefly, 60 μL of plasma or cytosolic fraction of liver was added to 1.80 mL of the reaction mixture, consisting of 0.83 mM 2,4,6-tripyridyl-s-triazine and 1.67 mM FeCl₃ × 6H₂O in 0.25 M acetate buffer (pH 3.6) with subsequent measurement of the change in absorption at 593 nm for 4 min.