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2. Nanoemulsion characterization

SP Sandra Milena Leal Pinto
LM Luis Alexandre Muehlmann
LO Lucía Liliana Mantilla Ojeda
AA Angélica María Vera Arias
MC Martha Viviana Roa Cordero
MS María de Fátima Menezes Almeida Santos
RA Ricardo Bentes Azevedo
PR Patricia Escobar Rivero
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The droplet size, polydispersity index (PDI) and zeta potential were measured by dynamic light scattering (DLS) and electrophoretic laser Doppler velocimetry using a Zetasizer Nano ZS® (Malvern Instruments, Malvern, UK). Samples were diluted 1:50 (v:v) in PBS before readings. All measurements were performed in triplicate, and the results are presented as the mean ± SD. The pH was assessed with an indicator strip (Merck, Darmstadt, Germany, 0 - 14). The physicochemical stability of the NEs was evaluated each month for at least four months after preparation. Samples were stored at 4°C, and PDI, zeta potential and ROS generation after PDT were measured following the protocols described above.

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