4.8. Curcumin Internalization in Macrophages Isolated and Infected with Leishmania

The process of curcumin internalization started with the culture and infection of macrophages performed in glass coverslips arranged in 24 well plates. The internalization of curcumin at 31.3, 15.6, and 7.8 µg/mL was evaluated. The incubation time was one hour, followed by washing with PBS and fixing with 4% paraformaldehyde for 15 min at room temperature. After fixing, the coverslips were again washed with PBS and mounted on glass slides. The analysis was performed under a Zeiss LSM 700 confocal microscope.