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GUS staining of DR5:GUS plants was performed using 50 mM sodium phosphate buffer (pH 7.0) containing 0.5% (v/v) Triton X-100, 1 mM K3/K4 FeCN, 0.05% (w/v) EDTA, and 1 mg/ml X-Gluc (Duchefa). At least, 10 seedlings and plants per genotype were fixed 5 min in 90% (v/v) acetone at −20°C and after this at 37°C overnight. Tissue was cleared with 70% (v/v) ethanol, and photographs were taken using a LEICA MZ 16 FA microscope coupled to a LEICA DFC 490 digital camera.
Copyright and License information: ©2021 Sánchez-Vicente, Lechón, Fernández-Marcos, Sanz and Lorenzo.
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